Comparative evaluation of PCR and imprint smear microscopy analyses of skin biopsy specimens in diagnosis of macular, papular, and mixed papulo-nodular lesions of post-kala-azar dermal leishmaniasis.
نویسندگان
چکیده
Diagnosis of post-kala-azar dermal leishmaniasis (PKDL), particularly the macular form, is difficult when based on microscopy. This study compared the results of nested PCR (91.9% positive samples) with imprint smear microscopy (70.9% positive samples) for 62 PKDL samples. We found that nested PCR, which indicated 87.5% positivity for the macular lesions, compared to 41.6% positivity by imprint smear microscopy, is an efficient method for early diagnosis of PKDL.
منابع مشابه
Comparative Evaluation of PCR and Imprint Smear Microscopy of Skin
27 Diagnosis of Post Kala-azar dermal leishmaniasis (PKDL), particularly the macular form is 28 difficult by microscopy. This study compared the positivity of nested polymerase chain reaction 29 (91.9%) with imprint smear microscopy (70.9%) in 62 PKDL samples. Nested PCR, showing 87.5% 30 positivity in macular lesions compared to 41.6% by imprint smear microscopy, is an efficient method 31 for ...
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Post-kala-azar dermal leishmaniasis (PKDL) is a dermatologic manifestation that usually occurs after visceral leishmaniasis (VL) caused by Leishmania donovani. It is characterized by hypopigmented patches, a macular or maculopapular rash and nodular skin lesions on the body surface. Involvement of the mucosae is very rare and unusual in PKDL. We report a case of PKDL that presented with polymor...
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Post-kala-azar dermal leishmaniasis (PKDL) is an important factor in kala-azar transmission; hence its early detection and assessment of effective treatment is very important for disease control. In present study on 60 PKDL cases presented with macular, mixed papulonodular, or erythematous lesions, Leishmania parasites were demonstrated microscopically in 91% of papulonodular and 40% of macular...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 51 12 شماره
صفحات -
تاریخ انتشار 2013